lc3b (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc lc3b
Lc3b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lc3b/LC3B+XP+Rabbit+mAb/pm42874772-95-50-53
Average 96 stars, based on 215 article reviews

lc3b - by Bioz Stars, 2026-11

96/100 stars

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Cell-Signaling:

Article Title: SGLT2 inhibition induces autophagic flux blockade and sensitizes pancreatic cancer to EGFR-targeted therapy
Article Snippet: After incubation with HRP-conjugated secondary antibodies (Abclonal) for 1 h at room temperature, signals were detected using the enhanced chemiluminescence (ECL) reagent. .. The following primary antibodies were used: SGLT2 (Invitrogen, PA5-101893, 1:1000; #14120, CST, 1:1000), Phospho-AMPKα (Thr172; #2535, Cell Signaling Technology, CST, Danvers, MA, USA; 1:1000), AMPKα1/α2 ( A27099 , Abclonal, Wuhan, China; 1:2000), Phospho-ULK1 (Ser555, #5869, CST; 1:1000), ULK1 (#8054, CST; 1:1000), SQSTM1/p62 (#5114, CST; 1:1000), LC3B (#3868, CST; 1:1000), Bax (#2772, CST; 1:1000), Bcl-2 (#3498, CST; 1:1000), Cleaved Caspase-3 (#9661, CST; 1:1000), Caspase-3 (#14220, CST; 1:1000), EGFR (A11351, Abclonal; 1:2000), and Phospho-EGFR (Tyr1068, AP0994, Abclonal; 1:1000; #3777, CST, 1:1000). .. β-Actin (AC026, Abclonal; 1:80000) was used as the loading control.

Article Title: Identification of Pinostilbene as a natural STING agonist that triggers FTH1 degradation via K48-ubiquitination to induce ferroptosis in non-small cell lung cancer
Article Snippet: Antibodies against STING, TBK1, and IRF3 were purchased from Proteintech (Wuhan, China). .. Phospho-STING (p-STING), phospho-TBK1 ( p -TBK1), phospho-IRF3 ( p -IRF3), LC3B, IRP1, and IRP2 antibodies were obtained from Cell Signaling Technology (CST, Danvers, MA, USA). .. Anti-Nuclear receptor coactivator 4 (NCOA4) antibody was purchased from Abcam (Cambridge, UK).

Western Blot:

Article Title: Cucurbitacin B attenuates LPS-induced skeletal muscle atrophy by inhibiting JAK2/STAT3 and suppressing proteasome activity.
Article Snippet: .. Main primary antibodies used for Western blotting: MyoD (Novus Cat. no. NBP1–54153), MyHC (Abcam Cat. no. ab37484), p-IGF-1R (CST, Cat. no. 3918 T), IGF-1R (CST, Cat. no. 9750 T), IRS-1 (CST, Cat. no. 3407 T), p-Akt (CST, Cat. no. 4060),Akt (SC, Cat. no. Sc-5298), p-mTOR (CST, Cat. no. 2971), mTOR (SC, Cat. no. Sc-8319), p-GSK3β (CST, Cat. no. 5558), GSK3β (SC, Cat. no. Sc-53,931), p-p70S6K (CST, Cat. no. 9204), p70S6K (SC, Cat. no. Sc-8418), p-4E-BP1 (CST, Cat. no. 9451), 4E-BP1 (SC, Cat. no. Sc-6936), FoxO3a (CST, Cat. no. 12829S), Lamin B (Absin Cat. no. Abs131244), Atrogin-1 (Novus Cat. no. NBP1–79517), MuRF1 (SC, Cat. no. Sc-398,608), LC3B (CST, Cat. no. 43566 T), Beclin1 (CST, Cat. no. 3495), P62 (Abmart, Cat. no. T55546F), β-actin(Absin Cat. no. abs32001), p-STAT3 (Tyr 705) (CST, Cat. no. 9145), p-STAT3 (Ser 727) (CST, Cat. no. 9134), STAT3 (SC, Cat. no. Sc-8019), p-JAK1 (CST, Cat. no. 3331), JAK1 (CST, Cat. no. 3332), p-JAK2 (CST, Cat. no. 3776), JAK2 (CST, Cat. no. 34315), p-Src (CST, Cat. no. 2105), Src (CST, Cat. no. 2109), IL-1β (ABclonal,Cat. .. no. A16288),TNF-α (Abmart, Cat. no. PA2174F).

other:

Article Title: Colorectal cancer cells respond differentially to autophagy induced by tyrosine kinase inhibitors.
Article Snippet: The following antibodies were obtained from Cell signaling and are directed against: LC3B (# 3868S), Beclin1 (# 3738), SQSTM1/p62 (# 88588), Phospho-Ser235/236 S6 Ribosomal protein (# 2211S), S6 Ribosomal protein (# 2217S), Phospho-Ser371 p70S6 Kinase (# 9208), p70S6 Kinase (# 2708), Phospho-Thr359/Ser363 p90RSK (# 9344), p90RSK1/2/3 (# 9355), Phospho-Ser2448 mTOR (# 5536), mTOR (# 2983),

Incubation:

Article Title: Species-specific cleavage of the autophagy adaptor p62 dictates responses to TNF.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HALO image analysis platform v3.6.4134.263 Indica Labs https://indicalab.com/halo/ Area Quantification v2.4.2 algorithm Indica Labs https://indicalab.com/halo/ CytoNuclear v2.0.9 algorithm (HALO) Indica Labs https://indicalab.com/halo/ CRISPOR Concordet et al.59 https://crispor.gi.ucsc.edu Image Lab Touch Software v. 2.4.0.03 BioRad https://www.bio-rad.com ImageJ64 software ImageJ https://imagej.net/ij/ Incucyte Software v. 2024B Sartorius https://www.sartorius.com/en/products/ live-cell-imaging-analysis/live-cellanalysis-software/incucyte-softwarev2024b Other Orbitrap FusionTM LumosTM TribridTM Mass Spectrometer Thermo Fisher Scientific Cat# IQLAAEGAAPFADBMBHQ Easy-nLC 1200 LC System Thermo Fisher Scientific Cat# LC140 50 cm Emitters CoAnn Technologies Cat# ICT36007508-50 ABIRD - The Active Background Ion Reduction Device for nanospray LC/MS ESI Solutions Cat# ABIRD ll OPEN ACCESS Article e5 Molecular Cell 86, 1–13.e1–e11, April 16, 2026 membranes (Merck). .. Membranes were blocked with BSA 5% in TBST for 30 min and incubated with the following primary antibodies overnight (all 1:1000 dilution): ATG7 (Cell Signaling, Cat# 8558; RRID:AB_11179039), BiP (Cell Signaling, Cat# 3177; RRID:AB_2119845), β actin (Cell Signaling, Cat# 4970; RRID:AB_2223172), Caspase-2 (BD Transduction Laboratories, Cat# 611023; RRID:AB_397606), Caspase-8 (Cell Signaling, Cat# 4790; RRID:AB_10545723), Caspase-9 (Cell Signaling, Cat# 9502; RRID:AB_2228052), CHOP (Cell Signaling, Cat# 2895; RRID:AB_2089254), Cleaved caspase-3 (Asp175) (Cell Signaling, Cat# 9664; RRID:AB_2070042), Cleaved caspase 7 (Asp198) (Cell Signaling, Cat# 9491; RRID:AB_2068261), Cleaved caspase-8 (Cell Signaling, Cat# 9429; RRID:AB_2062634), Cleaved caspase-8 (Asp374) (for human samples) (Cell Signaling, Cat# 9496S; RRID:AB_561378), Cleaved caspase-8 (mouse specific) (Cell Signaling, Cat# 8592S; RRID:AB_10999739), Cleaved PARP (Asp214) (rodent specific) (Cell Signaling, Cat# 94885; RRID:AB_2800237), FADD (Abcam, Cat# 124812; RRID:AB_11155370), FADD (Merck, Cat# 05-486; RRID:AB_309984), FLAG (Sigma-Aldrich, Cat# F7425; RRID:AB_439687), FLAG (M2) mAb (Merck, Cat# F3165; RRID:AB_2209044), GAPDH (Abcam, Cat# ab9484; RRID:AB_307274), GFP (Santa Cruz Biotechnology, Cat# sc-9996; RRID:AB_627695), HA-tag (Merck, Cat# 11867423001; RRID:AB_390918), HSP90 (Santa Cruz, Cat# sc-1057; RRID:AB_675714), LC3B (Cell Signaling, Cat# 3868; RRID:AB_2137707), MLKL (Cell Signaling, Cat# 14993; RRID:AB_2687572), p62 in human cells (BD Transduction Laboratories, Cat# 610833; RRID:AB_398152), p62 in mouse cells (Sigma-Aldrich, Cat# P0067; RRID:AB_1841028), p-MLKL (Cell Signaling, Cat# 91689; RRID:AB_2732034), pRIPK1 S166 (rodent specific) (Cell Signaling, Cat# 31122; RRID:AB_2799000), p-RIPK1 (Ser166) (Cell Signaling, Cat# 65746; RRID:AB_2799693), RIPK1 (BD Transduction Laboratories, Cat# 610459; RRID:AB_398668), TRADD (Abcam, Cat# ab319029; RRID: AB_3741122), TRADD (Cell Signaling, Cat# 3694; RRID:AB_2209044). .. Vinculin (Sigma-Aldrich, Cat# V9131; RRID:AB_477629).

Article Title: Gandouling protects against hepatic fibrosis in Wilson disease through the lncRNA-SNHG7/miR-29b/DNMT3A pathway.
Article Snippet: Proteins were diluted 1:4 in 5X SDS-PAGE sample buffer (Solarbio, Item No. G8200), followed by boiling in a water bath for 15 min. After cooling to room temperature, the samples were separated on SDS-PAGE, transferred to PVDF membranes (Millipore, Item No.IPVH00010), and blocked with 5% skim milk powder. .. The membranes were then incubated overnight with antibodies against DNMT3A (Bioss, Item No. bs-23029R, 1:2000, Rabbit, 1:1000, Rabbit), p-DNMT3A (Bioss, Item No. bs-14399R, 1:2000, Rabbit), α-SMA (Bioss, Item No. Bsm-33187 M, 1:2000, Mouse; No. bs-0189R, 1:1000, Rabbit), Collagen I (Bioss, Item No. bs-7158R, 1:1000, Rabbit; No. AB260043, 1:1000, Rabbit), Beclin-1 (Abcam, Item No. ab210498, 1:2000, Rabbit; No. ab62472, 1:1000, Rabbit), LC3B (CST, Item No. 3868s, 1:1000, Rabbit; No. 43566 S, 1:1000, Rabbit), and p62 (Bioss, Item No. bs-2951R, 1:1000, Rabbit) at 4°C with gentle shaking. .. HRP-labeled secondary antibodies (1:20,000) were then incubated with blots for 1.2 h, followed by three washes with PBST (10 min/wash) and development with an ECL Ultra Sensitive Luminescence Kit (Thermo, Item No. 340958).

Gentle:

Membrane:

Article Title: Targeting GHSR-1α with silibinin to enhance mitophagy and prevent tubular injury in diabetic kidney disease.
Article Snippet: Diabetic kidney disease (DKD) remains a leading cause of global renal failure, with tubular injury driven by mitochondrial dysfunction representing a critical yet therapeutically unaddressed mechanism.. Although the flavonolignan silibinin has demonstrated renoprotective potential, its precise molecular targets and mechanisms in DKD have remained unclear.. In this study, we combined integrated computational docking, biophysical assays, and comprehensive in vivo and in vitro models to investigate silibinin's interaction with the growth hormone secretagogue receptor-1α (GHSR-1α).

Review